>6202211 // ???? ACGATTCTCAGAT // start of pRSETB (@67) ATTTTGTTTACT ^ second A missing TTAAGAAGGA [-RBS-] GATATACAT // ORF starts here ATGCGG GGTTCTCATCATCATCATCATCATGGTATGGCTAGCATGACTGGTGGACA GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATC // end of pRSETB (@197) CCGCCACCA TGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATG CGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGAT CGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGC TGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCC CCTCAGTTCATGGCCGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACAT CCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCG TGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCC CTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTT CCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCT CCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAG CAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGAC CACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCA ACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAA CAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAG CTGTACAAGTAA // ORF ends here // pRSETB resumes (@226, 29 bp were spliced out) GAATTCGAAGCTTGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTG CCACCGCTGAGCAATAACTAG [T7 rev priming site] CATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGACGCG CCCTGTAACG ^ should be G GCGCATAAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACC ^ should be A TTGGCAGCGC CCTAACGCCC GTTCTTTCCA TTTCTTCCTT TCTTTTCTCG CCCGTTCCGC GGCTTTCCCG TCAAGCTTAA ATCGGGGGTT CCTTTAGGGT CCAATTATGG TTTACGGCAC TCGACCCAAA AAATTGGTAA GGGGAAGGTC AAGTATAGGG CATGCCCCTG AGAACGATTT TCAC